Zingiberensis Newsaponin Inhibits the Malignant Progression of Hepatocellular Carcinoma via Suppressing Autophagy Moderated by the AKR1C1-Mediated JAK2/STAT3 Pathway

Figure 4

ZnS suppresses autophagosome formation in HCC cells. (a) Monodansylcadaverine (MDC) staining used for the assessment of autophagy in Huh7 and SMMC7721 cells. Bar = 100 µm. (b) Transmission electron microscope utilized for the detection of autophagosomes in Huh7 and SMMC7721 cells. Bar = 2 µm. Cells were treated with 0.5 µM ZnS for Huh7 and 1.0 µM for SMMC7721 cells and/or 10 mM 3-methyladenine (3-MA) for 48 h.  < 0.001 vs. the control group.  < 0.001 vs. the ZnS + 3-MA group.

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