Anti-Inflammatory Effects of the Fraction from the Leaves of Pyrus pyrifolia on LPS-Stimulated THP-1 Cells

Figure 3

Effects of PP on mRNA expression of proinflammatory molecules in LPS-stimulated THP-1 cells. THP-1 cells were pretreated with PP (5–20 μg/mL) or DEX (20 μM) for 1 h; then cells were maintained with 0.1 μg/mL LPS for 6 h. The total RNA was isolated, and the mRNA levels encoding TNF-α, MCP-1, IL-8, and IL-6 were determined by RT-PCR (a). Relative mRNA expression was calculated using β-actin as a general housekeeping gene (b). Data are expressed as the mean ± SD. versus negative control group; and versus LPS only group.

(a)

(b)