Anti-Inflammatory Effects of the Fraction from the Leaves of Pyrus pyrifolia on LPS-Stimulated THP-1 Cells

Figure 5

Effects of PP on LPS-induced NF-κB transcriptional activity in THP-1 cells. THP-1 cells were pretreated with PP (5–20 μg/mL) for 1 h; then cells were maintained with 0.1 μg/mL LPS for 1 h. The levels of IκB-α and p65 phosphorylation and IκB-α degradation were detected with whole-cell lysates by western blot analysis (a). The levels of NF-κB translocation were detected by western blot separated into the cytosol and nucleus (b). Data are expressed as the mean ± SD. versus negative control group; and versus LPS only group.

(a)

(b)