Oridonin Dose-Dependently Modulates the Cell Senescence and Apoptosis of Gastric Cancer Cells

Figure 2

Oridonin induced cell cycle arrest in GC cells. (a) Colonies of MGC803 and AGS cells after treated with low-dose oridonin. Data are expressed as mean ± SD (n = 3; P < 0.01; P < 0.001 (vs. 0 μM); ^###P < 0.001 (vs. 5 μM)). (b) AGS cells maintained in medium containing gradient concentrations of oridonin for 24 h. The cell cycle distribution is analyzed by flow cytometric assay. (c) Quantitative analysis of cell cycle distribution. Data are presented as the mean ± SD of three separate experiments. P < 0.01; P < 0.001 (vs. 0 μM); ^#P < 0.05 (vs. 3 μM) (d) AGS cells treated with oridonin for 24 h cells and subjected to Western blot analysis. The expression levels of CDK4, CDK6, and p21 were analyzed; β-actin is used as a protein-loading control.

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