Oridonin Dose-Dependently Modulates the Cell Senescence and Apoptosis of Gastric Cancer Cells

Figure 5

AP4 overexpression reversed the oridonin-induced senescence of GC cells. (a) AGS cells are infected with AP4 virus or control virus by addition of virus into the cell culture at MOI of 100. The proliferation of AGS cells that overexpressed AP4 or the negative control treated with or without oridonin is determined. P < 0.01 and P < 0.001. The P values are analyzed by two-way ANOVA. (b) AGS cells that infected with AP4virus or control virus are treated with or without oridonin, Western blot analysis is performed to examine the expression of AP4, p53, and p21 in cells. β-Actin is used as a protein-loading control. (c) AGS cells infected with AP4 virus or control virus are treated with or without oridonin for 48 h and then maintained cells with oridonin-free medium for additional 4 days. β-gal-positive cells are identified by senescence-associated β-galactosidase activity assay. (d) Quantitative analysis of senescent cells with the percentage of β-gal-positive cells per unit area (n = 3, bar graphs are plotted as the mean ± SD, p < 0.01).

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