Oridonin Dose-Dependently Modulates the Cell Senescence and Apoptosis of Gastric Cancer Cells

Figure 6

High-dose oridonin induced GC cell apoptosis. (a) HGC27 and AGS cells treated with oridonin for 24 h. The cells were stained with 7-AAD and PE Annexin V. Apoptotic cells were identified using flow cytometry. Data are presented as the mean ± SD of three independent experiments (p < 0.05, p < 0.01, p < 0.001 (vs. 0 μM); ^#p < 0.05 (vs. 5 μM)). (b) Effects of oridonin on the expression of apoptotic-associated proteins. MGC803 and AGS GC cells were treated with oridonin for 24 h. Western blot analysis was carried out with antibodies against cleaved PARP, caspase-3, and cleaved caspase-3. β-Actin was used as a protein-loading control.

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