Study of the Antimalarial Activity of the Leaf Extracts and Fractions of Persea americana and Dacryodes edulis and Their HPLC Analysis
Table 4
Parasitemia, survival time, body weight, and PCV of infected mice treated with the extracts and fractions in the four-day suppressive test.
Group
Treatment dose (mg/kg)
Parasitemia level
Mean survival time (days)
Body weight (g)
PCV (%)
% parasitemia
% suppression
Day 0
Day 4
% change
Day 0
Day 4
% change
1
400 mg/kg PA
7.50 ± 0.65
55.00 ± 0.06
6.60 ± 1.08
12.44 ± 1.44
10.64 ± 0.52
−14.47 ± 0.06
41.00 ± 1.00
37.25 ± 0.75
−9.15 ± 0.03
2
200 mg/kg PAH
7.33 ± 0.88
56.03 ± 0.07
6.00 ± 1.52
11.84 ± 0.91
11.10 ± 0.80
−6.25 ± 0.04
39.00 ± 0.71
37.01 ± 0.58
−5.10 ± 0.02
3
200 mg/kg PAE
10.00 ± 0.58
40.00 ± 0.05
5.80 ± 1.69
15.48 ± 2.89
12.75 ± 2.08
−17.64 ± 0.01
38.20 ± 0.66
39.67 ± 1.45
3.85 ± 0.03
4
200 mg/kg PAM
13.00 ± 1.41
22.02 ± 0.09
10.6 ± 2.73
14.10 ± 2.27
12.36 ± 1.62
−12.34 ± 0.03
38.80 ± 0.86
39.25 ± 0.63
1.16 ± 0.04
5
200 mg/kg PAW
6.01 ± 0.91
64.01 ± 0.08
17.6 ± 3.67
13.82 ± 1.32
12.16 ± 1.10
−12.01 ± 0.01
39.40 ± 0.60
37.75 ± 0.63
−4.19 ± 0.02
6
400 mg/kg DE
6.33 ± 0.33
62.03 ± 0.01
10.20 ± 2.97
19.76 ± 1.47
20.80 ± 0.48
5.26 ± 0.03
40.00 ± 0.32
36.33 ± 2.19
−9.18 ± 0.02
7
200 mg/kg DEH
7.75 ± 1.25
53.51 ± 0.09
10.40 ± 4.15
16.88 ± 0.69
21.70 ± 2.71
28.55 ± 0.07
39.40 ± 1.08
36.50 ± 1.70
−7.36 ± 0.12
8
200 mg/kg DEE
7.00 ± 0.58
58.01 ± 0.06
8.0 ± 4.29
19.36 ± 0.60
21.50 ± 0.15
11.05 ± 0.03
38.20 ± 0.66
34.00 ± 0.58
−10.99 ± 0.05
9
200 mg/kg DEM
4.67 ± 0.88
71.99 ± 0.06
11.60 ± 5.07
17.32 ± 1.02
19.78 ± 0.94
12.44 ± 0.02
40.60 ± 0.51
34.00 ± 0.58
−16.26 ± 0.04
10
ACT
5.01 ± 0.58
70.00 ± 0.06
16 ± 1.14
17.52 ± 0.47
20.05 ± 0.52
14.44 ± 0.02
39.40 ± 0.51
39.67 ± 0.33
0.69 ± 0.02
11
Control
16.67 ± 1.45
—
4.8 ± 0.66
18.22 ± 1.69
14.90 ± 1.22
−18.22 ± 0.07
40.00 ± 0.71
36.67 ± 1.76
−8.33 ± 0.08
PA = P. americana leaf crude extract, PAH = P. americana hexane fraction; PAE = P. americana ethyl acetate fraction; PAM : P. americana methanol fraction; PAW =P. americana water fraction; DE = D. edulis leaf extract; DEH = D. edulis hexane fraction; DEE = D. edulis ethyl acetate fraction; DEM = D. edulis methanol fraction. DEW (D. edulis water fraction) was not tested due to its limited quantity.
