Prediction of Rhizoma Drynariae Targets in the Treatment of Osteoarthritis Based on Network Pharmacology and Experimental Verification

Figure 11

NF-κB p65 and IκB-α levels in IL-1β-treated SW1353 cells pretreated with the aqueous extract of Rhizoma Drynariae (AERD) and luteolin. SW1353 cells were pretreated with 2500 mg/L AERD or 30 μmol/L luteolin for 1 h and then treated with 10 ng/mL IL-1β for 30 min. The IκB-α level in the cytoplasm and the NF-κB p65 level in the nucleus were measured by western blotting. Lamin B and β-actin were used as internal references for the nuclear and cytoplasmic fractions, respectively. The data are derived from three independent experiments and expressed as the mean ± standard deviation ( < 0.05: increased when compared to the control group; ^# < 0.05: decreased when compared to the IL-1β-treated group; and  < 0.05: decreased when compared with the AERD-treated group).

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