Prediction of Rhizoma Drynariae Targets in the Treatment of Osteoarthritis Based on Network Pharmacology and Experimental Verification

Figure 15

mRNA levels of HIF-1α and TNF-α in IL-1β-treated SW1353 cells pretreated with the aqueous extract of Rhizoma Drynariae (AERD) or luteolin. SW1353 cells were pretreated with 2500 mg/L AERD or 30 μmol/L luteolin for 1 h and then treated with 10 ng/mL IL-1β for 12h. (a) mRNA expression of HIF-1α in IL-1β-treated SW1353 cells pretreated with AERD or luteolin. (b) mRNA expression of TNF-α in IL-1β-treated SW1353 cells pretreated with AERD or luteolin. The data are derived from three independent experiments and expressed as mean ± standard deviation ( < 0.05: increase compared to the control group; ^# < 0.05: decrease compared to the IL-1β-treated group; and ^△ < 0.05: decrease compared with the AERD-treated group).