Research Article
Auraptene, a Monoterpene Coumarin, Inhibits LTA-Induced Inflammatory Mediators via Modulating NF-κB/MAPKs Signaling Pathways
Figure 3
Effects of AU in LTA-induced phosphorylation of MAPKs in RAW 264.7 macrophages. (a) Cells were untreated or pretreated with AU (5 and 10 μM) for 30 min and were then stimulated with LTA (5 μg/ml) for 1 h. The specific pERK, pJNK, and p38 MAPK antibodies were used to detect these proteins. α-Tubulin was used as the internal control. (b)–(d) The statistical values shown are the means ± S.E.M. of four independent experiments. and vs. the control cells; and vs. LTA-stimulated cells.
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