Auraptene, a Monoterpene Coumarin, Inhibits LTA-Induced Inflammatory Mediators via Modulating NF-κB/MAPKs Signaling Pathways

Figure 4

AU controls the NF-κB signaling pathway induced by LTA in RAW 264.7 macrophages. Cells were pretreated with AU (5 and 10 μM) for 30 min and were then stimulated with LTA (5 μg/ml) for 1 h. The phosphorylation of (a) IκBα and (b) p65 in LTA-induced RAW cells was detected as described in Section 2. (c) PTE inhibited LTA-induced p65 nuclear translocation. The values shown are the means ± S.E.M. of four independent experiments. and vs. the control cells; , , and vs. LTA-stimulated cells.

(a)

(b)

(c)