Research Article
Auraptene, a Monoterpene Coumarin, Inhibits LTA-Induced Inflammatory Mediators via Modulating NF-κB/MAPKs Signaling Pathways
Figure 5
AU enhances antioxidant defense molecules in LTA-stimulated RAW cells. Cells were untreated or pretreated with AU (5 and 10 μM) for 30 min followed by LTA (5 μg/ml) for 24 h. The expression of HO-1 (a), catalase (CAT) activity (b), and glutathione (GSH) content (c) in LTA-induced RAW cells was determined using western blotting, native polyacrylamide gel electrophoresis (NATIVE-PAGE), and spectrophotometric analyses, respectively. The values shown are the means ± S.E.M. of four independent experiments. vs. the control cells.
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