A Comparative Study on Anticancer Effects of the Alhagi maurorum and Amygdalus haussknechtii Extracts Alone and in Combination with Docetaxel on 4T1 Breast Cancer Cells

Figure 5

AO/EB staining assay. (a) Negative control group: the circular nucleus distributed in the center of the cell. (b) DTX treated group: nucleus showed granular appearance with yellow-green fluorescence by AO staining indicating apoptosis. (c) A.m (IC50): nucleus of the cells showed orange fluorescence indicating late apoptosis. (d) A.h (IC50): the nucleus of cells showed yellow-green fluorescence and located in bias. (e) A.m + DTX (IC50 + IC50) : yellow-green fluorescence was observed in the nucleus of cells. (f) A.m + DTX (IC50 + IC10) : apoptotic vesicles were observed out of the cells clearly. (g) A.m + DTX (IC25 + IC25) : the dominant red color by EB staining indicated necrosis and they were disintegrating. (h) A.m + DTX (IC10 + IC10): late-stage apoptotic cells were detected with concentrated and asymmetrically localized orange nuclear EB staining. (i) A.h + DTX (IC50 + IC50), (j) A.h + DTX (IC50 + IC10), and (l) A.h + DTX (IC10 + IC10): apoptotic cells with nucleus showed yellow-green fluorescence and were located in one side of cells. (k) A.h + DTX (IC25 + IC25): the dominant red color indicated necrosis. IC values were as follows: for A.m : IC10 = 32 µg/ml, IC25 = 40 µg/ml, and IC50 = 57 µg/ml; for A.h : IC10 = 10 µg/ml, IC25 = 33 µg/ml, and IC50 = 85 µg/ml; and for DTX : IC10 = 0.4 µM, IC25 = 3.5 µM, and IC50 = 11 µM.

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