Sendeng-4 Suppressed Melanoma Growth by Induction of Autophagy and Apoptosis

Figure 5

Sendeng-4 promote cell autophagy. A375 and A875 cells were treated with different concentrations of Sengdeng-4 for 12 h. (a) Western blot analysis was performed to measure protein levels of LC3I/II, beclin-1, and p62. ß-Actin was used as an internal control. (b, c) Quantification of (a). (d, f) Immunofluorescence was performed to measure cell autophagy. (f, g) Quantification of (d).

(a)

(b)

(c)

(d)

(e)

(f)

(g)