Kochia scoparia Saponin Momordin Ic Modulates HaCaT Cell Proliferation and Apoptosis via the Wnt/β-Catenin Pathway

Figure 4

Momordin Ic acts on HaCaT cells through the Wnt/β-catenin signaling. Target cells were treated with PBS, 30 mM LiCl, 25 μmol/L Momordin Ic + PBS, or 25 μmol/L Momordin Ic + 30 mM LiCl for 48 h and examined for cell viability by MTT assay (a); cell apoptosis and cell cycle by flow cytometry (b-c); the mRNA expression of β-catenin, c-myc, and VEGF by qRT-PCR (d); the protein levels of β-catenin, c-myc, and VEGF by immunoblotting (e); the content and distribution of β-catenin by immunofluorescent (IF) staining (f) (scale bar is 20 μm). , .

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