Strong Twirling-Rotating Manual Acupuncture with 4 r/s Is Superior to 2 r/s in Relieving Pain by Activating C-Fibers in Rat Models of CFA-Induced Pain

Figure 3

The activation of primary sensory neurons in the DRG by twirling-rotating MA with different frequencies (n = 3). (a) The representative images of C‐FOS (+) co-labeled with CGRP (+) in DRG among each group; (b) comparison of the proportion of CGRP‐positive neurons excited by twirling-rotating MA with two forms of frequencies. (c) The representative images of C‐FOS(+) co‐labeled with NF200(+) in DRG among each group; (d) comparison of the proportion of NF200‐positive neurons excited by twirling‐rotating MA with two forms of frequencies. Red represents C‐FOS; green represents NF200 or CGRP. The white arrow indicates examples of co-labeled neurons. The bar = 50 um. ; , compared with the control group; ; , compared with the 2 r/s MA group.

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