Strong Twirling-Rotating Manual Acupuncture with 4 r/s Is Superior to 2 r/s in Relieving Pain by Activating C-Fibers in Rat Models of CFA-Induced Pain

Figure 4

The activation of the DRG neurons and acupuncture analgesia of MA with different frequencies affected by RTX (n = 5). (a, b) The representative images of C‐FOS co-labeled with CGRP-positive neurons and C‐FOS co‐labeled with NF200‐positive neurons in the DRG among each group. The white arrows indicated examples of co-labeled neurons. Bar = 50 um. (c, d) Comparison of the proportion of CGRP-positive neurons and NF200‐positive neurons between the 4 r/s MA, 4 r/s MA+ RTX, 2 r/s MA, 2 r/s MA + RTX, and CFA groups. (e). The analgesic effects of 2 forms of MA affected by RTX. The white arrow indicates examples of co-labeled neurons. ; , compared with the control group; ; , compared with the CFA group. ; , compared with the CFA + 4 r/s MA + RTX group. , compared with the CFA + 2 r/s MA + RTX group.

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