Inhibitory Effect of Phellinus baumii Extract on CFA-Induced Inflammation in MH-S Cells through Nuclear Factor-κB and Mitogen-Activated Protein Kinase Signaling Pathways

Figure 2

Effect of PBE on CFA-stimulated proinflammatory mediators and cytokines in MH-S macrophages analyzed using reverse transcription polymerase chain reaction (RT-PCR). (a) The mRNA levels of proinflammatory mediators (iNOS and COX-2) and proinflammatory cytokines (IL-1β, IL-6, and TNF-α) measured after 18 h of CFA (2.5 μg/mL) incubation and GAPDH utilized as a housekeeping gene in RT-PCR. (b–f) The levels of protein expression densitometrically analyzed using ImageJ software. A 6-well plate is used to seed the cells, and the PBE doses of 12.5, 25, 50, and 100 μg/mL are used. All the values from three independent experiments are expressed as standard error of the mean. in comparison to the basal group. , , and in comparison to the CFA group.

(a)

(b)

(c)

(d)

(e)

(f)