Inhibitory Effect of Phellinus baumii Extract on CFA-Induced Inflammation in MH-S Cells through Nuclear Factor-κB and Mitogen-Activated Protein Kinase Signaling Pathways

Figure 3

Effect of PBE on CFA-stimulated mRNA expression in MH-S macrophages measured using real-time polymerase chain reaction (PCR). (a–e) The mRNA levels of iNOS, COX-2, IL-1β, IL-6, and TNF-α are measured by quantitative real-time PCR after 18 h of CFA (2.5 μg/mL) incubation. GAPDH is used as a control gene. A 6-well plate is used to seed the cells, and PBE doses of 12.5, 25, 50, and 100 μg/mL are used. All the values from three independent experiments are expressed as standard error of mean. in comparison to the basal group. , , and in comparison to the CFA group.

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