Inhibitory Effect of Phellinus baumii Extract on CFA-Induced Inflammation in MH-S Cells through Nuclear Factor-κB and Mitogen-Activated Protein Kinase Signaling Pathways

Figure 4

Effect of PBE on CFA-stimulated nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) pathways in MH-S macrophages. (a) The protein levels of p-IκB, phosphorylated NF-κB, and NOD-like receptor protein 3, and the MAPK (extracellular signal-regulated kinase, p38, and c-Jun N-terminal kinase) pathway investigated using Western blot after 18 h of CFA (2.5 μg/mL) incubation. As a loading control, β-actin was used. (b–g) The expression levels of protein were densitometrically analyzed using ImageJ software. A 6-well plate is used to seed the cells, and the PBE doses of 12.5, 25, 50, and 100 μg/mL are used. All the values from three independent experiments are expressed as standard error of the mean. in comparison to the basal group. , , and in comparison to the CFA group.

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