Inhibitory Effect of Phellinus baumii Extract on CFA-Induced Inflammation in MH-S Cells through Nuclear Factor-κB and Mitogen-Activated Protein Kinase Signaling Pathways

Figure 5

Effect of PBE on CFA-stimulated nuclear factor-kappa B (NF-κB) translocation in MH-S macrophages. The cells are seeded on a coated cover slip in a 6-well plate and grouped as follows: basal, CFA (2.5 μg/mL)-induced, CFA with PBE (100 μg/mL), and CFA with Bay-11, inhibitor of phosphorylated (p-NF-κB) groups. The cells are treated with PBE and Bay-11 (10 μM) for 30 min prior to the CFA treatment (2.5 μg/mL) and incubated for 18 h. Immunofluorescence staining is used to examine p-NF-κB nuclear translocation. To visualize the nuclei, the samples are mounted using a Prolong Gold Antifade reagent with DAPI (blue). Confocal microscopy (Zeiss) at ×400 original magnification is used to examine stained cells.