Duchesnea indica Extract Attenuates Coal Fly Ash-Induced Inflammation in Murine Alveolar Macrophages through the NF-Kappa B Pathway

Table 1

Primer used for RT-PCR and real-time PCR analysis.

RT-PCR	Forward primer sequences (5′–3′)	Reverse primer sequences (5′–3′)
COX-2	CACTACATCCTGACCCACTT	ATGCTCCTGCTTGAGTATGT
iNOS	CCCTTCCGAAGTTTCTGGCAGCAGC	GGCTGTCAGAGCCTCGTGGCTTTGG
IL-6	GTACTCCAGAAGACCAGAGG	TGCTGGTGACAACCACGGCC
TNF-α	TTGACCTCAGCGCTGAGTTG	CCTGTAGCCCACGTCGTAGC
IL-1β	CTGTGGAGAAGCTGTGGCAG	GGGATCCACACTCTCCAGCT
GAPDH	CACTCACGGCAAATTCAACGGCAC	GACTCCACGACATACTCAGCAC
Real-time PCR
COX-2	GGCAGCCTGTGAGACCTTTG	GCATTGGAAGTGAAGCGTTTC
iNOS	GGCAGCCTGTGAGACCTTTG	GCATTGGAAGTGAAGCGTTTC
IL-6	TCCAGTTGCCTTCTTGGGAC	GTGTAATTAAGCCTCCGACTTG
TNF-α	TGCCTATGTCTCAGCCTCTTC	GAGGCCATTTGGGAACTTCT
IL-1β	CAACCAACAAGTGATATTCTCCATG	GATCCACACTCTCCAGCTGCA
GAPDH	CACTCACGGCAAATTCAACGGCAC	GACTCCACGACATACTCAGCAC