Induction of Apoptosis in HeLa Cells by a Novel Peptide from Fruiting Bodies of Morchella importuna via the Mitochondrial Apoptotic Pathway

Figure 4

Nuclear morphological changes induced by MIPP in HeLa cells determined via TUNEL assay. (a) Cells cultured under standard conditions (negative control). (b) Cells treated with resveratrol (50 μM) as the positive control. (c), (d), and (e) Cells treated with different concentrations (50, 100, and 200 μg/mL) of MIPP. (f) Semiquantitative analysis of TUNEL positive area of HeLa cells from immunofluorescence staining. The HeLa cells were cultivated under standard conditions for 12 h before treatment with different concentrations (50, 100, and 200 μg/mL) of MIPP and resveratrol (50 μM) for 12 h 4′,6-diamidino-2-phenylindole (DAPI) excites blue fluorescence in the nuclei of cells. TUNEL reaction mixture shows apoptotic cells via red fluorescence. In the superimposed image, the nuclei of apoptotic cells exhibit a light purple fluorescence. The arrow in the figure points to the nuclei of apoptotic cells. Scale bar = 50 μm. The semiquantitative analysis was carried by Image Pro Plus 6.0 software (Media Cybernetics, Maryland, USA) and values with different superscript letters were significantly different from each other at . All experiments were repeated three times.

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