Curcumin Ameliorates Palmitic Acid-Induced Saos-2 Cell Apoptosis Via Inhibiting Oxidative Stress and Autophagy

Figure 1

Effects of curcumin on PA-induced cell viability and apoptosis in Saos-2 cells. Saos-2 cells were treated with different concentrations of curcumin for 24 h. Cell viability was measured using CCK-8 assay (a). Cells were incubated with 200 μM PA for 24 h with or without curcumin at different concentrations. Cell viability was measured using CCK-8 assay (b). Apoptosis analysis was performed via flow cytometry (c). The protein expression levels of Bcl-2 and BAX were detected by western blot analysis. The analysis of band intensities is presented as the relative ratio of BAX to Bcl-2 (d). The Caspase-3 activity was detected using a microplate reader (e). Ctrl, control; Cur, different concentrations of curcumin; PA, 200 mM palmitic acid. Statistical analysis is shown on the bar graphs. Data are presented as the mean ± SEM of the three independent experiments. versus control; versus PA.

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