Curcumin Ameliorates Palmitic Acid-Induced Saos-2 Cell Apoptosis Via Inhibiting Oxidative Stress and Autophagy

Figure 2

Effects of curcumin on PA-induced oxidative stress and autophagy in Saos-2 cells. Saos-2 cells were incubated with 200 μM PA for 24 h with or without curcumin at different concentrations. The generation of ROS was measured by using the DCFH-DA. Saos-2 cells were stained with DCFH-DA and analyzed by fluorescent microscopy. Statistical analysis of fluorescence intensity in Saos-2 cells by image J software (a). The relative levels of SOD were detected by a microplate reader (b). Western blot detected ATG5, P62, and LC3 (c). Ratio of ATG5 and P62 to GAPDH, and ratio of LC3- II to LC3- I (d, e, and f). Ctrl, control; Cur, different concentrations of curcumin; PA, 200 mM palmitic acid. Statistical analysis is shown on the bar graphs. Data are presented as the mean ± SEM of the three independent experiments. versus control; versus PA.

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