Curcumin Ameliorates Palmitic Acid-Induced Saos-2 Cell Apoptosis Via Inhibiting Oxidative Stress and Autophagy

Figure 5

Curcumin attenuated oxidative stress-induced Saos-2 cell apoptosis. Saos-2 cells were incubated with H₂O₂ or AY-22989 for 24 h with or without curcumin. Cell viability was measured using CCK-8 assay (a). Apoptosis analysis was performed via flow cytometry using Annexin V-FITC/PI double staining (b, c). The protein expression levels of Bcl-2 and BAX were detected by western blot analysis. The analysis of band intensities is presented as the relative ratio of BAX to Bcl-2 (d). The Caspase-3 activity was detected using a microplate reader (e). Ctrl: control; Cur: 10 µM curcumin; H₂O₂: 100 µM H₂O₂; AY: 10 µM AY-22989. Statistical analysis is shown on the bar graphs. Data are presented as the mean ± SEM of the three independent experiments. versus control, versus PA.

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