Astragali Radix Contributes to the Inhibition of Liver Fibrosis via High-Mobility Group Box 1-Mediated Inflammatory Signaling Pathway

Figure 4

Effect of AR on HMGB1/TLR4/NF-κB signaling pathway in liver fibrosis induced by CCl₄. (a–e) Immunohistochemistry analysis for the effect of AR on HMGB1 localization (original magnification, ×400; n = 3; (a) control group; (b) model group; (c) 2.7 g/kg AR group; (d) 5.4 g/kg AR group; (e) 10.8 g/kg AR group). (f) The mean integrated optical density of HMGB1. (g) HMGB1 mRNA level. (h) TLR4 mRNA level. (i) Myd88 mRNA level. (j) RAGE mRNA level. (k) Western blot images of NF-κB p65. (l) NF-κB p65 protein level. Values are expressed as mean ± SD (n = 6). and when compared with the control group. and when compared with the model group.

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