Curcumin Inhibits HGF-Induced EMT by Regulating c-MET-Dependent PI3K/Akt/mTOR Signaling Pathways in Meningioma

Figure 2

The effects of curcumin on HGF-induced cell proliferation, apoptosis, invasion, migration, and epithelial-mesenchymal transition of meningioma IOMM-Lee cells. (a) IOMM-Lee cells were starved for 12 hours and then stimulated by 50 ng/ml of HGF in the presence of 2% FBS for 24, 48, and 72 hours. (b) IOMM-Lee cells were starved for 12 hours and then stimulated by 50 ng/ml of HGF in the presence of 2% FBS, and curcumin was added 4 hours before stimulation. Cell proliferation was detected by the CCK-8 assay at 24 and 48 hours after HGF stimulation. (c) IOMM-Lee cells were starved for 12 hours and then stimulated by 50 ng/ml of HGF in the presence of 2% FBS, and curcumin was added 4 hours before stimulation. Cell apoptosis was evaluated by the flow cytometry assay. (d) Statistical analysis result of apoptosis. The asterisks indicate significant differences. (e) IOMM-Lee cells were seeded into the upper chamber without FBS and invaded toward the lower chamber containing 2% FBS and 50 ng/ml HGF in the media (magnification: 100x). (f) Statistical analysis result of invasion. The asterisks indicate significant differences. (g) IOMM-Lee cells were starved for 12 hours and then stimulated by 50 ng/ml of HGF (with 2% FBS). Cell migration capability was determined by the wound healing assay (magnification: 100x). (h) Statistical analysis result of migration; the asterisks indicate significant differences. (i) IOMM-Lee cells were starved for 12 hours and then stimulated by 50 ng/ml of HGF (with 2% FBS). The expression of E-cadherin, N-cadherin, and vimentin was detected by western blot. (j) Statistical analysis result of E-cadherin, N-cadherin, and vimentin expression. Data are expressed as mean ± standard error; n = 3; the asterisk () indicates a significant difference compared to the ctrl group, , , , and . The hash (#) indicates a significant difference compared to the HGF group, ^#, ^##, ^###, and ^####.

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