Curcumin Inhibits HGF-Induced EMT by Regulating c-MET-Dependent PI3K/Akt/mTOR Signaling Pathways in Meningioma

Figure 3

Curcumin inhibits HGF-induced EMT via modulation of c-MET/PI3K/Akt/mTOR signaling pathways. (a) IOMM-Lee cells were starved for 12 hours and then stimulated by 50 ng/ml of HGF in the presence of 2% FBS, and curcumin was added 4 hours before stimulation. The protein expression of the c-MET/PI3K/Akt/mTOR marker was detected by western blot. (b) Statistical analysis result of c-MET/PI3K/Akt/mTOR marker protein expression. (c) IOMM-Lee cells were starved for 12 hours and then stimulated by 50 ng/ml of HGF in the presence of 2% FBS, and MET inhibitor SU11274 (5 μmol/l) or PI3K inhibitor LY294002 (25 μmol/l) was used 4 hours before HGF stimulation (magnification: 100x). (d) Statistical analysis result of the wound healing assay. (e) IOMM-Lee cells were seeded into the upper chamber without FBS and invaded toward the lower chamber containing 2% FBS and 50 ng/ml HGF in the media. MET inhibitor SU11274 (5 μmol/l) or PI3K inhibitor LY294002 (25 μmol/l) was used 4 hours before HGF stimulation (magnification: 100x). (f) Statistical analysis result of invasion. (g) IOMM-Lee cells were starved for 12 hours and then stimulated by 50 ng/ml of HGF in the presence of 2% FBS, and MET inhibitor SU11274 (5 μmol/l) or PI3K inhibitor LY294002 (25 μmol/l) was used 4 hours before HGF stimulation. (h) Statistical analysis result of invasion. The protein expression of EMT markers, i.e., vimentin, E-cadherin, and N-cadherin, was detected by western blot. Data are expressed as mean ± standard error; n = 3; the asterisk () indicates a significant difference compared to the ctrl group, , , , and . The hash (#) indicates a significant difference compared to the HGF group, ^#, ^##, ^###, and ^####.

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