Huganbuzure Granule Attenuates Concanavalin-A-Induced Immune Liver Injury in Mice via Regulating the Balance of Th1/Th2/Th17/Treg Cells and Inhibiting Apoptosis

<div>HBG inhibits hepatocyte apoptosis induced by JNK activation. (a, b) Serum levels of MDA and SOD. (c) Reactive oxygen species (ROS) fluorescence staining (×200) showing ROS levels in the liver. (d) Immunohistochemical staining (×200) showing the expression of p-JNK in liver tissue at 4 h after ConA injection. (e, f) The expression of p-p38 and p-ERK1/2 proteins was determined using western blot analysis, and gray values were calculated. (g) TUNEL staining (×200) showing apoptotic cells in five groups at 4 h after ConA injection. (h, i) The expression of cleaved caspase-3 protein was determined using western blot analysis, and gray values were calculated. <svg height="14.9569pt" id="M28" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -11.5275 53.9209 14.9569" width="53.9209pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,4.432,-5.741)"><path d="M457 455H370L403 612H360L326 455H218L252 612H210L175 455H77L68 394H161L132 257H31L20 196H117L77 19H120L159 196H268L230 19H272L311 196H402L412 257H325L355 394H447L457 455ZM313 394L283 257H175L204 394H313Z"></path></g><g transform="matrix(.013,0,0,-0.013,9.395,0)"><path d="M570 304C570 398 525 448 414 448C385 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Evidence-Based Complementary and Alternative Medicine

fig4

Figure 4

Figure 4: Huganbuzure Granule Attenuates Concanavalin-A-Induced Immune Liver Injury in Mice via Regulating the Balance of Th1/Th2/Th17/Treg Cells and Inhibiting Apoptosis 

Figure 4 | Huganbuzure Granule Attenuates Concanavalin-A-Induced Immune Liver Injury in Mice via Regulating the Balance of Th1/Th2/Th17/Treg Cells and Inhibiting Apoptosis 