Alkaloid Extract of Moringa oleifera Lam. Exerts Antitumor Activity in Human Non-Small-Cell Lung Cancer via Modulation of the JAK2/STAT3 Signaling Pathway

Figure 5

MOAE inhibits the activation of the JAK2/STAT3 signaling pathway in A549 cells. A549 cells were treated with MOAE (0, 100, or 200 μg/mL) for 48 h. (a) The protein expression of JAK2, p-JAK2, STAT3, and p-STAT3 was measured by western blotting assay, with β-actin used as the loading control. (b) Quantification of the relative levels of p-JAK2 and p-STAT3; each value was normalized to that of JAK2 and STAT3, respectively. Cell viability (c), cell apoptosis (d), and cell migration (e) were analyzed using the MTT assay, flow cytometry, and wound healing assay, respectively. A549 cells were pretreated for 2 h with AZD1480 (2.5 μM) before treatment with MOAE (0 or 200 μg/mL) for 48 h. The results are expressed as the mean ± SEM of three independent experiments. and vs. 0 μg/mL.

(a)

(b)

(c)

(d)

(e)