Extracts of Perilla frutescens var. Acuta (Odash.) Kudo Leaves Have Antitumor Effects on Breast Cancer Cells by Suppressing YAP Activity

Figure 3

PLE suppressed YAP target genes in a LATS1/2-dependent manner. (a) HEK293A cells were treated with increasing concentrations of PLE for 2 h. Cell lysates were immunoblotted using anti-LATS1 and anti-pLATS1 (S909) antibodies. Quantifications of western blots for pLATS1 (S909) were performed by ImageJ program. (b, c) Wild-type and LATS1/2 knockout (KO) HEK293A cells were treated with PLE. After 12 h, mRNA levels of CTGF and CYR61 were measured using RT-qPCR. Error bars represent ± SEM from n = 3 per group. (d) HEK293A cells were treated with increasing concentrations of PLE for 2 h. Cell lysates were immunoblotted using anti-MSTS1 and anti-pMST1 (T183) antibodies. (e, f) Wild-type and MST1/2 KO HEK293A cells were treated with PLE for 12 h and mRNA levels of CTGF and CYR61 were measured using RT-qPCR (error bars represent ± SEM from n = 3 per group). Cell lines were immunoblotted to analyze indicated antibodies.  < 0.05,  < 0.01, and  < 0.001; Student’s t-test (unpaired, one-tailed) was used for statistical analysis In Figure 3(a)), the numbers of size maker depart from indicated lines.

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