The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells

Figure 2

CE modulates the expression of cell cycle-related proteins in aHSCs. The aHSCs were treated with CE (1 mg/ml) and palmitic acid (PA; 75 μM) for 5 days. Western blot analysis was performed to detect the level of p27, cyclin D1, and β-actin. A quantitative western blot analysis was used to calculate the p27 and cyclin D1 values normalized to β-actin. The data are shown as the mean ± SD obtained from three independent experiments using isolated different batches of aHSCs. ^∗P < 0.05, ^∗∗P < 0.01, compared with the control (Ctrl).