The Effects of Freshwater Clam (Corbicula fluminea) Extract on Activated Hepatic Stellate Cells

Figure 6

CE modulates the expression of ECM-related genes in aHSCs. The aHSCs were incubated with or without CE (1 mg/ml) for 5 days. The RNA was isolated, and the expression levels of collagen I TIMP-II, MMP-9, TNF-α, and TGF-β1 were measured by qRT-PCR. The individual mRNA levels were normalized to constitutive GAPDH expression, and the fold changes in the mRNA levels of the target genes were calculated relative to the control. The data are shown as the mean ± SD obtained from three independent experiments using isolated from different batches of aHSCs. ^∗P < 0.05, ^∗∗P < 0.01, compared with the control.