Synergistic Cytotoxicity between Elephantopus scaber and Tamoxifen on MCF-7-Derived Multicellular Tumor Spheroid

Figure 4

Fluorescent microscopy observation of apoptosis in MCTS cultures of MCF-7 after dual staining with acridine orange and propidium iodide. The cultures were incubated for 4 days (a) without treatment or with treatment of (b) E. scaber ethanol extract 60 μg/mL (ES60), (c) tamoxifen citrate 3.125 μg/mL (TC3), (d) tamoxifen citrate 6.25 μg/mL (TC6), (e) ES60 + TC3, (f) ES60 + TC6 before subjected to acridine orange and propidium iodide staining.

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