Synergistic Cytotoxicity between Elephantopus scaber and Tamoxifen on MCF-7-Derived Multicellular Tumor Spheroid

Table 1

Sequence and properties of genes designed for real-time PCR gene expression study.

Gene		Primer sequence (5′–3′)	Tm (°C)	Product size (bp)	Accession no.
GAPDH	F	GGATTTGGTCGTATTGGGC	60.15	206	NM_002046.3
	R	TGGAAGATGGTGATGGGATT	60.13
18S rRNA	F	GATGCGGCGGCGTTATTC	58.50	120	X03205.1
	R	GTGGTGCCCTTCCGTCAA	56.20
β-actin	F	CCATCGTCCACCGCAAAT	57.34	308	NM_001101.3
	R	GACTTCCTGTAACAACGCATCT	58.85
c-Jun	F	CGACCTTCTATGACGATGCC	57.90	239	NM_002228.3
	R	CCCGTTGCTGGACTGGA	57.50
HSP 105	F	AGATGAAGCAGTAGCCAGAG	54.84	392	AF161368.1
	R	CCACCATAGATGCCGTAG	54.89
ICAM 1	F	GACCCCAACCCTTGATGATA	59.61	265	NM_000201.2
	R	GTGCTTTTGTGCCGATAGAA	58.92
VEGF	F	GCTGTGGACTTGAGTTGGG	55.60	195	NM_001204385.1
	R	GCTGGGTTTGTCGGTGTT	55.90

∗Tm—melting temperature, F—forward primer, R—reverse primer.