Ginsenoside Rg1 Alleviates Hepatic Ischemia-Reperfusion Injury in Mice via Activating ERα-Regulating YAP Expression

Figure 4

ERα expression profile in murine hepatic IRI; ERα activation alleviates liver IR injury. (a) Detection of ERα gene and protein level in IR mice at different time points after reperfusion, n = 3. (b) Mitochondrial ROS content, n = 6. (c) Mitochondrial JC-1 membrane potential of mouse liver, n = 6. (d) ATP content, n = 6. (e) Relative mRNA and protein expression of ERα, n = 3. (f) Serological ALT level, n = 6. (g) HE staining of liver tissue (×200; scale: 50 μm). Data are expressed in mean ± SEM; ^∗∗P < 0.01, ^∗∗∗∗P < 0.0001, versus sham group; ^#P < 0.05, ^####P < 0.0001, versus IR group; ^△△△△P < 0.001, ^△△△△P < 0.0001, versus E2 group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)