Yixintongmai Inhibits Proliferation and Migration and Promotes Apoptosis of Vascular Smooth Muscle Cells Cultured with High Glucose

Figure 4

Yixintongmai inhibits ROS generation and expression of NOX-1 of VSMCs cultured with high glucose. NG: normal glucose (5.5 mM); HG: high glucose (25 mM); HG + Y: high glucose (25 mM) and yixintongmai powder (360 μg/ml); MG: normal glucose (5.5 mM) and mannitol (19.5 mM). The intracellular ROS level shown by the fluorescence intensity of VSMCs under different conditions (a); densitometry quantification of mean fluorescence intensity values shown by the ratio of integrated density and area of VSMCs under different conditions (b); Western blot of expression of NOX-1 (c) and densitometry quantification (d); densitometry quantification values are shown by fold over NG or HG. Values are expressed as means ± SD of triplicate experiments. ; ; NS: not significant ().

(a)

(b)

(c)

(d)