Efficacy of Ethanolic Extract of Syzygium aromaticum in the Treatment of Multidrug-Resistant Pseudomonas aeruginosa Clinical Isolates Associated with Urinary Tract Infections

Table 3

Antibacterial screening of tested plant extracts (100 mg/mL) against the six clinical isolates of P. aeruginosa.

Tested plants

Inhibition effect diameter (mm)

S. aromaticum

C. longa

A. sativum

N. sativa

Z. officinale

Clinical isolates

H2O extract

EtOH extract

EtOAc extract

H2O extract

EtOH extract

EtOAc extract

H2O extract

EtOH extract

EtOAc extract

H2O extract

EtOH extract

EtOAc extract

H2O extract

EtOH extract

EtOAc extract

P4

7 ± 0.2

16 ± 0.1

14 ± 0.1

18 ± 0.2

9 ± 0.1

8 ± 0.1

—

—

—

—

—

—

—

—

—

Positive control

21 ± 0.36

20 ± 0.21

19 ± 1.6

14 ± 0.19

19 ± 0.04

P8

—

6 ± 0.2

15 ± 0.3

13 ± 0.6

10 ± 0.1

8 ± 0.2

—

—

—

—

—

—

—

—

—

Positive control

15 ± 0.31

15 ± 0.29

14 ± 0.11

15 ± 0.27

15 ± 0.14

P9

8 ± 0.5

23 ± 1.6

15 ± 0.8

12 ± 0.2

10 ± 0.1

7 ± 0.1

—

—

—

—

—

—

—

—

—

Positive control

23 ± 1.3

14 ± 0.8

11 ± 0.1

12 ± 0.3

9 ± 0.01

P12

12 ± 0.1

15 ± 0.3

13 ± 0.2

11 ± 0.1

8 ± 0.2

7 ± 0.1

—

—

—

—

—

—

—

—

—

Positive control

16 ± 1.1

12 ± 0.6

14 ± 0.26

9 ± 0.1

7 ± 0.06

P13

11 ± 0.3

17 ± 0.5

12 ± 0.1

10 ± 0.1

8 ± 0.1

8 ± 0.2

—

—

—

—

—

—

—

—

—

Positive control

18 ± 0.25

13 ± 0.36

15 ± 0.27

10 ± 0.14

8 ± 0.1

P14

10 ± 0.1

20 ± 0.6

13 ± 0.2

11 ± 0.1

9 ± 0.1

8 ± 0.1

—

—

—

—

—

—

—

—

—

Positive control

15 ± 1.3

13 ± 0.1

16 ± 0.6

14 ± 0.1

14 ± 0.3

Colistin (10 mg/disc); P4–P14: selected strains; —, no activity; inhibition zones including the diameter of the paper disc (6 mm). Data are means of three replicates (n = 3) ± standard error. It was confirmed that 10% DMSO had no inhibitory effect on any isolate.