Water-Soluble Total Flavonoids Isolated from Isodon lophanthoides var. gerardianus (Benth.) H. Hara Promote Hepatocellular Carcinoma Sensitivity to 5-Fluorouracil

Figure 13

NAC inhibits the impact of WSTF plus 5-FU on ROS accumulation, colony formation, and apoptosis-related protein expression within HepG2 cells. (a) After 48 h of incubation with WSTF and 5-FU with or without NAC, the ROS level in HepG2 cells was determined by DCFH-DA, and the relative intensity of average fluorescence is presented in the manner of mean ± SEM from 3 independent tests. (b) After 48 h of incubation with WSTF and 5-FU with or without NAC, after 1% crystal violet staining, each plate was photographed after 10 days. Colony percentage was determined and presented in the manner of mean ± SEM from 3 independent tests. (c) After 48 h of incubation with WSTF and 5-FU with or without NAC, the expression of apoptosis-related proteins by Western blotting was completed. .

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