Effects of Roasted Schisandra Chinensis (Turcz.) Baill and Lycium Chinense Mill. and Their Combinational Extracts on Antioxidant and Anti-Inflammatory Activities in RAW 264.7 Cells and in Alcohol-Induced Liver Damage Mice Model

Figure 1

The effects of raw or roasted Schisandra chinensis (Turcz.) Baill (SC), Lycium chinense Mill. (LC), or mass-based 1 : 1 mixture, SL, on LPS-stimulated nitric oxide (NO) production, ROS generation, and the protein expression of iNOX, COX-2, and NADPH oxidases (NOXs) in RAW264.7 macrophages. RAW264.7 cells were pretreated with raw or roasted (R) SC, LC, or SL (500 μg/mL) for 1 h and then incubated with LPS (1 μg/mL) for 18 h. (a–e) The culture media were collected for NO production, cell lysates were prepared for ROS generation, and iNOS, COX-2, NOX (−1, −2, and −4), and p22^phox, p47^phox, and p67^phox levels were analyzed by immunoblotting. The densitometry data represented are shown as the relative density of protein bands normalized to β-actin level. vs. blank B; , , vs. control (C: LPS treatment group). ^ǂ vs. RSC, RLC, and SL. Values are the mean ± SEM of three independent experiments.

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