Effects of Roasted Schisandra Chinensis (Turcz.) Baill and Lycium Chinense Mill. and Their Combinational Extracts on Antioxidant and Anti-Inflammatory Activities in RAW 264.7 Cells and in Alcohol-Induced Liver Damage Mice Model

Figure 5

Suppression of inflammatory mediators and MAPK and NF-κB signaling in alcohol-fed mice livers by SL and RSL. (a) TNF-α and (b) IL-1β levels were analyzed using ELISA kit in liver lysates. Phosphorylation of (c) ERK1/2, JNK, and p38, and (d) NF-κB and IκB was determined by Western blot analysis in lysed liver tissue. The total NF-κB, IκB, ERK1/2, JNK, or p38 staining is shown as a loading control, respectively. The quantitative results are depicted. The densitometry data represent the relative density of protein bands normalized to β-actin level. , , vs. N; , , vs. C. ^ǂ vs. SL. N: normal group; C: ethanol-fed control group; SL: ethanol-fed group with SL treatment; RSL: ethanol-fed group with RSL. Values represent the mean ± SEM for 6 mice per group.

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