Glaucocalyxin A Attenuates Allergic Responses by Inhibiting Mast Cell Degranulation through p38MAPK/NrF2/HO-1 and HMGB1/TLR4/NF-<i>κ</i>B Signaling Pathways

<div>Effect of GLA on the secretion of proinflammatory cytokines and Fc<i>ε</i>RI-mediated signaling pathway in RBL-2H3 cells. RPMCs and RBL-2H3 cells were stimulated with anti-DNP IgE (50 ng/ml) for 6 h and challenged with DNP-HSA (100 ng/mL) with or without pretreatment with GLA (0.1, 1.0, and 5.0 <i>μ</i>M). Cytokine levels in the supernatants of RPMCs (a) and RBL-2H3 cells (b) were measured by ELISA. (c) The protein levels of Syk, Lyn, Fyn, Gab2, and PLC-<i>γ</i>1 and their phosphorylation were determined by Western blot. All data represent the mean ± SEM (<i>n</i> = 3). 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Evidence-Based Complementary and Alternative Medicine

fig3

Figure 3

Figure 3: Glaucocalyxin A Attenuates Allergic Responses by Inhibiting Mast Cell Degranulation through p38MAPK/NrF2/HO-1 and HMGB1/TLR4/NF-<i>κ</i>B Signaling Pathways 

Figure 3 | Glaucocalyxin A Attenuates Allergic Responses by Inhibiting Mast Cell Degranulation through p38MAPK/NrF2/HO-1 and HMGB1/TLR4/NF-κB Signaling Pathways 