HPLC Quantification of Thymoquinone Extracted from Nigella sativa L. (Ranunculaceae) Seeds and Antibacterial Activity of Its Extracts against Bacillus Species

Table 1

Qualitative analysis of phytoconstituents in the N. sativa seed extracts.

Sr. no.	Phytochemicals	Methanol extract	Ethanol extract	Hexane extract	Ether extract	Aqueous extract
1	Alkaloids	++	−	+	++	−
2	Carbohydrates	−	+	−	+	−
3	Flavonoids	++	++	−	++	+
4	Glycosides	++	+	−	−	−
5	Phlobatannins	−	−	−	−	−
6	Proteins	−	−	−	−	−
7	Reducing sugars	−	−	−	−	−
8	Saponins	++	−	+++	−	+
9	Tannins	+	−	+	−	+
10	Terpenoids	+++	+	+++	+	−
11	Vitamin C	+++	+++	+++	+++	+++

⁺Presence of phytochemicals. ⁻Absence of phytochemicals. ⁺⁺Moderate concentration. ⁺⁺⁺High concentration.