HPLC Quantification of Thymoquinone Extracted from Nigella sativa L. (Ranunculaceae) Seeds and Antibacterial Activity of Its Extracts against Bacillus Species

Table 2

Antibacterial activity of N. sativa extracts using well diffusion assay.

Tested bacteria	Concentration (mg/µL)	Methanol extract	Ethanol extract	Hexane extract	Ether extract	Aqueous extract
	Inhibition zones (mm)
B. subtilis	1.25	9	8	11	N.I.	8
	2.5	21	13	17	19	13
	5	37	28	41	35	26
B. licheniformis	1.25	7	7	N.I.	N.I.	N.I.
	2.5	26	21	19	10	11
	5	30	22	40	40	30

N.I.: no inhibition.