HPLC Quantification of Thymoquinone Extracted from <i>Nigella sativa</i> L. (<i>Ranunculaceae</i>) Seeds and Antibacterial Activity of Its Extracts against <i>Bacillus</i> Species

<table class="table-group" id="tab4"><tr><td><table class="table"><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr class="thead"><td class="align_left" rowspan="2">Tested bacteria</td><td class="align_center">Kalonji oil (commercial)</td><td class="align_center">Commercial oil in methanol (5 mg/<i>µ</i>L)</td><td class="align_center">Kalonji oil local vendor</td><td class="align_center">Local oil in methanol (5 mg/<i>µ</i>L)</td><td class="align_center">Isolated thymoquinone</td></tr><tr class="thead"><td class="align_center" colspan="5">Inhibition zones (mm)</td></tr><tr><td class="thead-hr" colspan="6"><hr/></td></tr><tr><td class="align_left"><i>B. subtilis</i></td><td class="align_center">25</td><td class="align_center">32</td><td class="align_center">30</td><td class="align_center">31</td><td class="align_center">44</td></tr><tr><td class="align_left"><i>B. licheniformis</i></td><td class="align_center">21</td><td class="align_center">22</td><td class="align_center">19</td><td class="align_center">20</td><td class="align_center">38</td></tr><tr class="table-tr"><td colspan="6"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>Antimicrobial activity of the oil samples.</div>

Evidence-Based Complementary and Alternative Medicine

tab4

Table 4

Table 4: HPLC Quantification of Thymoquinone Extracted from <i>Nigella sativa</i> L. (<i>Ranunculaceae</i>) Seeds and Antibacterial Activity of Its Extracts against <i>Bacillus</i> Species 

Table 4 | HPLC Quantification of Thymoquinone Extracted from Nigella sativa L. (Ranunculaceae) Seeds and Antibacterial Activity of Its Extracts against Bacillus Species 