HPLC Quantification of Thymoquinone Extracted from <i>Nigella sativa</i> L. (<i>Ranunculaceae</i>) Seeds and Antibacterial Activity of Its Extracts against <i>Bacillus</i> Species

<table class="table-group" id="tab6"><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr class="thead"><td class="align_left" rowspan="2"><i>ƛ</i> (nm)</td><td class="align_center" colspan="3">Absorbance</td></tr><tr class="thead"><td class="align_center">265</td><td class="align_center">253</td><td class="align_center">250</td></tr><tr><td class="thead-hr" colspan="4"><hr/></td></tr><tr><td class="align_left">1</td><td class="align_center">−0.039</td><td class="align_center">0.099</td><td class="align_center">−0.076</td></tr><tr><td class="align_left">2</td><td class="align_center">0.139</td><td class="align_center">0.154</td><td class="align_center">−0.386</td></tr><tr><td class="align_left">3</td><td class="align_center">−0.039</td><td class="align_center">0.098</td><td class="align_center">−0.033</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

<div>The absorbance of the fractions obtained by column chromatography of 80% methanolic extract in the UV spectra.</div>

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Table 6

Table 6: HPLC Quantification of Thymoquinone Extracted from <i>Nigella sativa</i> L. (<i>Ranunculaceae</i>) Seeds and Antibacterial Activity of Its Extracts against <i>Bacillus</i> Species 

Table 6 | HPLC Quantification of Thymoquinone Extracted from Nigella sativa L. (Ranunculaceae) Seeds and Antibacterial Activity of Its Extracts against Bacillus Species 