Da Cheng Qi Decoction Alleviates Cerulein-Stimulated AR42J Pancreatic Acinar Cell Injury via the JAK2/STAT3 Signaling Pathway

Figure 6

The amelioration of DCQD was mediated by suppressing the activation of JAK2/STAT3 signaling pathway. (a and f) The morphological changes of cells were assessed by using an optical microscope (400×). The releases of amylase, lipase, and C-reactive protein were detected by amylase assay kit (b and g), lipase assay kit (c and h), and C-reactive protein assay kit (d and i), respectively. (e) Western blot was performed to detect the protein level of JAK2, p-JAK2, STAT3, and p-STAT3. GAPDH was as the loading control for band density normalization. (j) Western blot was performed to detect the protein level of STAT3 and p-STAT3. GAPDH was as the loading control for band density normalization. Data are presented as the means ± SD, n = 3. Micrographs from one representative experiment out of three independent experiments are shown. All bar graphics:  < 0.05,  < 0.01 vs. the model group.

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