Xiaoyaosan Exerts Therapeutic Effects on the Colon of Chronic Restraint Stress Model Rats via the Regulation of Immunoinflammatory Activation Induced by the TLR4/NLRP3 Inflammasome Signaling Pathway

Figure 8

The effects of XYS on the mRNA and protein expression of TLR4, MyD88, NF-κB-p65, TAK1, IRAK1, TRAF6, NLRP3, caspase-1, and ASC in the rat colon. (a) The effects of XYS on the mRNA expression levels of Tlr4, Myd88, NF-κB-p65, Tak1, Irak1, Traf6, Nlrp3, caspase-1, and Asc in the rat colon. For Tlr4, Myd88, NF-κB-p65, Tak1, Irak1, and Traf6, data are expressed as the means ± SD of 5 rats per group. and vs. the model group; ^▲ and ^▲▲ vs. the control group. For Nlrp3, caspase-1, and Asc, data are expressed as the means ± SD of 3 rats per group. and vs. the model group; ^▲ and ^▲▲ vs. the control group. (b)-(c) Semiquantitative Western blot analysis of TLR4, MyD88, NF-κB-p65, TAK1, IRAK1, and TRAF6 expression in colonic tissue; data are expressed as the means ± SD of 5 rats per group. ^▲ and ^▲▲ vs. the control group; and vs. the model group. Semiquantitative Western blot analysis of NLRP3, ASC, and caspase-1 expression in colonic tissue; data are expressed as the means ± SD of 3 rats per group. ^▲ and ^▲▲ vs. the control group; and vs. the model group. TLR4, Toll-like receptor 4; MyD88, myeloid differentiation primary response protein; IRAK1, interleukin 1 receptor-associated kinase 1; TAK1, transforming growth factor beta-activated kinase 1; TRAF6, tumor necrosis factor receptor-associated factor 6; NF-κB-p65, nuclear factor kappa beta-p65; NLRP3, NOD-like receptor protein 3; ASC, apoptosis-associated speck-like protein; XYS, Xiaoyaosan; FLU, fluoxetine.

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