Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway

Table 1

Primers used for qRT-PCR analysis.

Gene name	Prime name	Primers (5′ to 3′)	Length
GAPDH	Forward	TGACAACTTTGGCATCGTGG	78
	Reverse	GGGCCATCCACAGTCTTCTG
HIF-1α	Forward	AGCAATTCTCCAAGCCCTCC	91
	Reverse	CGGTGGCAGTGACAGTGAT
Caspase-3	Forward	TCTACCGCACCCGGTTACTA	88
	Reverse	CGTACAGTTTCAGCATGGCG
Beclin-1	Forward	CCCAGCCAGGATGATGTCTAC	96
	Reverse	AGTCTCCGGCTGAGGTTCTC
Bcl-2	Forward	ACTCTTCAGGGATGGGGTGA	94
	Reverse	TGACATCTCCCTGTTGACGC
BNIP3	Forward	ATTGGTCAAGTCGGCCAGAA	81
	Reverse	TCATGCTGAGAGTAGCTGTGC
LC3	Forward	TGGTGGAAGAGACGAAAGCC	76
	Reverse	ACCAGCATCGTAGAGGGTCT
Atg5	Forward	CAAGCAGCTCTGGATGGGAC	140
	Reverse	AAAGGCCGTTCAGTTGTGGT
P62	Forward	GTGGAACCCCAGTAAGAGGC	115
	Reverse	TGAATACCAGCTGTCCGAGC